Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline | MFCD00131855 | 5 x 10 pkg
Phosphate buffered saline | MFCD00131855 | 5 x 10 pkg
Alkali Scientific CellPro™ Tris Buffered Saline 10X, pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Teknova 1M Tris-HCl, pH 8.0, 0.5mL, 50 Tubes per Pack, Sterile. 6 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Teknova 1M Tris-HCl, pH 7.5. 500mL, Sterile, DNAse/RNAse Tested 8 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Cambridge Isotope Laboratories CDC BFR PAR Solution (unlabeled) 1000 ng/mL in nonane 1 2 mL
CDC BFR PAR Solution (unlabeled) 1000 ng/mL in nonane 1 2 mL
Alkali Scientific CellPro™ Tris Buffered Saline 10X, pH 7.4, 1L
Alkali Scientific™ CellPro™ Tris solution offers sterile and filtered Tris buffer for various laboratory applications. This high-quality solution is meticulously prepared to ensure purity and consistency, meeting the stringent requirements of scientific research and experimentation. With its sterile formulation, it minimizes the risk of contamination, making it suitable for a wide range of cell culture and molecular biology procedures. The filtered nature of the solution ensures clarity and reliability in experimental results, providing researchers with confidence in their findings. Whether used in protein biochemistry, DNA electrophoresis, or other molecular biology techniques, CellPro™ Tris solution delivers reliable performance and consistent results, contributing to the success of laboratory workflows.
Bioworld TBE Buffer (25X), 4 L
Product DescriptionTBE (Tris-Borate-EDTA) has a liquid concentrate of 25X, where 1X contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. 1L of concentrate makes 25L of 1X buffer. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Biotang Inc TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
TAE (50X PH 8.0) 1000ML. BM-250. 2 M Tris-base, 1M acetate and 50 mM EDTA, pH 8.0 +/- 0.2. Prepared in 18.2 megohms water and filtered through 1 micron filter.
Zymo Research Corporation DNA/RNA Wash Buffer (Concentrate), 24 ml
DNA/RNA Wash Buffer (Concentrate) (24 ml). For use in Quick-DNA™/RNA purification kits. Sold individually or as part of a nucleic acid purification kit.