Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules Methyl (2-(Hydroxymethyl)Phenyl)Carbamate | 117550-36-4 | MFCD14680132 | 250mg
WuXi AppTec | Methyl (2-(Hydroxymethyl)Phenyl)Carbamate | 250mg | 583186989 | LN01746856 | | 117550-36-4 | MFCD14680132 | 181.191 | C9H11NO3
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Boston Bioproducts Inc Tris-Glycine-SDS Running Buffer 10X, 4 L, 8.50 ± 0.20 pH, 250 mM Tris, 1.92 M Glycine, 1% SDS, Anode and Cathode buffer, SDS-PAGE, Western blotting
This Tris-Glycine SDS Running Buffer (10X) is specifically formulated for use in protein electrophoresis applications, such as SDS-PAGE and Western blotting. It plays a vital role in the separation and resolution of denatured proteins. Upon dilution to the recommended working concentration, the buffer ensures consistent results with well-resolved protein bands. Furthermore, this buffer can function effectively as both the anode and cathode buffer, maintaining the pH essential for optimum electrophoresis performance.
- Volume: 4 L
- Concentration: 10X
- pH: 8.50 ± 0.20
- Application: SDS-PAGE, Western blotting
- Function: Separation of denatured proteins
- Recommended dilution: 10-fold with deionized water for 1X use
- Storage conditions: Room temperature
- Filtered through: 1-micron filter
- Quality control testing: Appearance, pH
Bioworld Tris-Glycine-SDS (TGS) Running Buffer (10X), 4 L
Product DescriptionTris-Glycine SDS (TGS) running buffer has a concentration of 10X with a pH of 8.3. 1X solution contains 0.025M Tris, 0.192M Glycine, and 0.1% SDS. Product is of ultra pure grade.TG buffers are blended using pure reagents to provide high quality for protein research. This buffer is formulated to ensure exceptional purity along with the absence of nuclease and protease activities. TG buffer is widely used for electroblotting proteins in Western blot procedures and is recommended for use with all nitrocellulose and nylon membranes.
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 1L, 1 Liter, 10/Case
CellPro™ PBS Buffer, 1X pH 7.2, 1 Liter Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Kd Medical Inc PBS BUFFER, 1X, PH 7.2
Standard buffer for many biomolecular procedures. One 1 Liter bottle.
Growcells TAE BUFFER 50X 1L EA
Sterile liquid electrophoresis buffer concentrate prepared with 18.2 megOhm water, 0.2 micron sterile filtered and steam sterilized in final packaging. Recipe/L: 2424.8g TRIS Base, 570ml Acetic Acid, 186.1g EDTA Ph 8.3
Seracare Life Sciences Inc 10X DULBECCO S PBS (D-PBS)
10X Phosphate-buffered Saline (PBS) is ideal for use in sample preparation and as a wash buffer in general immunoassay applications as well as tissue and cell culture procedures. This modified Dulbecco's buffer is not formulated with magnesium or calcium salts. pH=7.4. Conductivity controlled.