Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Sigma Aldrich Fine Chemicals Biosciences PIPES | 5625-37-6 | MFCD00006159 | 100g
PIPES | 99% | MW: 307.32 | 5625-37-6 | MFCD00006159 | 100g
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
Electron Microscopy Sciences Tris Buffered Saline, pH 7.4 250 ML
C₄H₁₁NO₃ F.W. 121.14
Our buffers are ideal for maintaining a constant pH during fixation and have an ionic constitution to prevent the extraction of cellular components or the deposition of precipitates of fixative chemicals. The buffers are prepared fresh for each order and conveniently packaged.
pH: 7.4
Sigma Aldrich Fine Chemicals Biosciences Trizma base | 77-86-1 | MFCD00004679 | 100g
Trizma base | Purity: 99.9% | Mol Wt: 121.41 | 77-86-1 | MFCD00004679 | 100g
Research Products International Corp HEPES, Sodium Salt, 5 KG
pKa (20°C): 7.45-7.65
pH Range: 6.8-8.2
Sigma Aldrich Fine Chemicals Biosciences 5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | 90217-02-0 | MFCD00467521 | 10MG
5-Cyano-2,3-di-(p-tolyl)tetrazolium chloride >=85% (HPLC) | Purity: >=85% (HPLC) | Mol Wt: 311.77 | 90217-02-0 | MFCD00467521 | 10MG
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.6) - 500ml
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.0) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging.
Sigma Aldrich Fine Chemicals Biosciences HEPES solution 1 M pH 7.0
HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH the molecule is zwitterionic and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.